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. 2022 Nov 30;11(23):3848. doi: 10.3390/cells11233848

Figure 5.

Figure 5

Perk-Apob48 interaction is required for FA/choline-mediated CMs assembly. (A) Western blot analysis for Mtp, Apob48, and Sar1b in the IECs under FA and CH incubation and transfected with perk siRNA. (B) Quantification analysis for Western blot. (C) mRNA levels of apob, mtp, sar1b genes. (D) Relative MTP activity. (E) The structural protein prediction model between Perk and Apob48. (F) IP analysis of Perk-Apob48 complex. Data are mean ± SEM, n = 3 independent biological experiments; different lower-case letters indicate significant differences in si-NC groups; different capital letters indicate significant differences in si-perk groups (p ≤ 0.05); asterisks indicate significant differences between si-NC and si-perk groups (* p ≤ 0.05).