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. 2022 Nov 24;14(23):5781. doi: 10.3390/cancers14235781

Figure 2.

Figure 2

WNT7B and its receptor FZD4 are activated by large hepatitis B surface antigens (L-HBs). (A,B) qPCR analysis of WNT7B mRNA levels following pCH9/3091 or pUC18-HBV1.3 transfection. (C,D) The WNT7B protein level was investigated using a Western blotting test in response to pCH9/3091 or pUC18-HBV1.3 transfection. (E,F) WNT7B expression was measured using qPCR (E) and Western blotting (F) after the transient transfection of six HBV structural proteins (L-HBs, S-HBs, HBc, HBe, HBx, HBp). (G) The STRING bioinformatics tool was used to predict WNT7B interacting proteins. (H,I) FZD4 expression was measured by qPCR (H) and Western blotting (I) following WNT7B overexpression. (J,K) FZD4 expression was determined by qPCR (J) and Western blotting (K) following WNT7B knockdown. (L,M) The FZD4 expression level was determined by qPCR (L) and Western blotting (M) after L-HBs and S-HBs overexpression. All graphs depict the mean ± SD of at least three independent experiments. * p < 0.05, ** p < 0.005, *** p < 0.001, **** p < 0.0001.