FIG. 4.

SWP1 is a single-copy gene present in at least three Encephalitozoon species. (A) Genomic DNA from 1.5 × 10⁸ E. cuniculi organisms was digested with a panel of different restriction enzymes, separated on a 0.7% agarose gel, blotted onto nylon membranes, hybridized with a ³²P-labeled SWP1 cDNA fragment, and washed under high-stringency conditions (0.2× SSC [1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate]–0.1% SDS at 65°C). The restriction pattern is in accordance with SWP1 being a single-copy gene. (B) Genomic DNA from E. cuniculi, E. hellem, and E. intestinalis was digested with BamHI/HindIII and hybridized as described in panel A, followed by washing under moderate stringency (0.2× SSC–0.1% SDS at 45°C).