FIG. 6.

SWP1 is not modified by glycosaminoglycans. (A) A total of 7 × 10⁶ freshly harvested E. cuniculi organisms were digested with heparinase I, heparinase III, or chondroitase ABC and subjected to immunoblot analysis using MAb 11A1. (B) The pellet of 7 × 10⁶ E. cuniculi organisms was incubated for 12 h with 0.3 M NaBH₄ in 0.4 M NaOH in order to release O-linked glycosaminoglycans by alkaline hydrolysis. Samples were subjected to immunoblot analysis using MAb 11A1. The molecular size of SWP1 from treated samples is identical to that from untreated samples, indicating that glycosaminoglycan residues are not attached to the protein.