Figure 1.
Animal feeding groups and experimental timeline. 0.3 mg Cr/kg of body weight as chromium picolinate (Cr-Pic) and chromium nanoparticles (Cr-NPs) were added to the diet as an emulsion together with dietary rapeseed oil for 9 weeks of supplementation after the initial 9 week period of experimental feed without Cr supplementation. Rats at 7 weeks of age were fed with two types of diet: a standard-fat-and-fiber (S) diet and a high-fat–low-fiber (F) diet for either 9 weeks or 18 weeks (9 + 9 weeks). The following groups of rats were studied: Control S: the S diet plus the S diet; Control FS: the F diet and the S diet; Cr–Pic FS: the F diet and the Cr–Pic S diet; Cr–NPs FS: the F diet and the Cr–NPs S diet; Control FF: the F diet and the F diet; Cr–Pic FF: the F diet and the Cr–Pic F diet; Cr–NPs FF: the F diet and the Cr–NPs F diet.
