Figure 2. L1 expression in cerebella is the same regardless of treatment group.
A) Representative immunoblot of L1 from cerebella of rat pups under the 5 treatment conditions. The blot shows two bands, the 220 kD band representing the full length form of L1, and the 80 kD band representing the cleaved transmembrane-intracellular domain. By convention, the 80 kD band is used to quantify L1 due to the difficulty in transferring the full length L1. B) Representative immunoblot of β–actin from cerebella of rat pups under the 5 treatment groups. The blot shows a single band at 42 kD representing β–actin. C) Bar Graph: Mean +/− SEM of the ratio of total L1 to β–actin (n=3 pups per treatment group from 3 different litters). There was no significant difference using ANOVA with (p=0.97469) or without (p=0.94669) the control group.