FIGURE 3.
Cysteine restriction, but not methionine restriction, induces hepatic de novo serine biosynthesis. CR, but not MR, increased hepatic serine concentrations (a), Phgdh mRNA (b, c), and Phgdh protein levels (d, e). For statistics and annotations refer to Figure 1. In panel a, n = 16 for CD and SAAR groups, n = 8/group for all other groups; in all other panels, n = 4–8/group; in panel d, only the diets with the highest and lowest concentration of Met (MR1 and MR4) and Cys (CR1 and CR5) were probed. μc—cannot determine (due to the absence of bands, numbers for band densities were not available)