FIG. 6.

RT-PCR analysis for the expression of P6-specific cytokine mRNA by MEM T cells. RNA preparations from MEM T cells of P6+CT-immunized mice incubated with P6 contained mRNA for Th1 and Th2 cytokines, including IFN-γ, IL-5, IL-6, IL-10, and TGF-β. Control cells were CD4⁺ T cells of P6+CT-immunized mice incubated without P6. Each analysis was performed at least three times to verify the results. Lanes: 1, β-actin (540 bp); 2, IFN-γ (365 bp); 3, IL-2 (413 bp); 4, IL-4 (357 bp); 5, IL-5 (424 bp); 6, IL-6 (638 bp); 7, IL-10 (455 bp); 8, TGF-β (525 bp).