Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 10;13:7658. doi: 10.1038/s41467-022-35424-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a SA-β-Gal positivity was analyzed in human OA chondrocytes after 3 and 4 weeks of culture with tgg2-PP-MDL-800 NP or control (5, 10, or 20 µM). n = 6 independent biological replicates per group. b Representative immunofluorescent images of indicated markers in human OA chondrocytes after 4 weeks of culture with tgg2-PP-MDL-800 NP or control (5, 10, or 20 µM). n = 6 independent biological replicates per group. c Proteoglycan content was measured by safranin O-fast green staining in human OA cartilage explants after 3 and 4 weeks of incubation with tgg2-PP-MDL-800 NP. n = 6 independent biological replicates per group. d Western blot were performed to determine the Col II expression level at 3 and 4 weeks. n = 3 independent biological replicates per group. e Overview of the experimental set-up with injections of PBS, tgg2-PP NP or tgg2-PP-MDL-800 NP. f Representative immunofluorescent images of IL-15, p-JAK3, and p-STAT5 levels in these chondrocytes from different treatment groups. n = 6 independent biological replicates per group. g Western blot analysis of protein levels of Acetyl, p-Stat5 (Y694), and Stat5. n = 3 independent biological replicates per group. h, i At 4 and 8 weeks, Safranin O-fast green stained sections and H&E of cartilage (medial femoral condyle) showed that OA phenotype was significantly alleviated in OA mice model treated by tgg2-PP-MDL-800 NP, as evaluated by OARSI. n = 6 mice per group (h). A high level of Col II was observed in the tgg2-PP-MDL-800 NP treatment group. n = 3 independent biological replicates per group (i). j At 4 and 8 weeks, synovial inflammation was evaluated. n = 6 mice per group. k Representative micro-CT images of subchondral bone in the medial tibial plateau of PBS, tgg2-PP NP, or tgg2-PP-MDL-800 NP-treated DMM mice at 4w and 8w. n = 6 mice per group. Scar bar: c 200 μm, h, j (upper) 50 μm, a, b, j (lower) 20 μm, f 10 μm. Data were presented as the mean ± s.e.m (a–d, i) or median (25–75th percentiles) (f, h, j). P values are from two-tailed unpaired Student’s t-test (a, control and 10 μM groups in c), two-tailed unpaired t-test with Welch’s correction (5 and 20 μM groups in c), one-way ANOVA test followed by Tukey’s post hoc (b), two-way ANOVA test followed by Tukey’s post hoc (d, i), Kruskal–Wallis test followed by Dunn’s post hoc (f) or Scheirer–Ray–Hare test followed by Dunn’s post hoc (h, j). Source data are provided as a Source Data file.