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. 2022 Dec 10;13:7656. doi: 10.1038/s41467-022-35417-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Schematic illustrating the intracellular C-tail domains of GluRIIA, GluRIIB, and the chimeric GluRIIB subunit substituted with the GluRIIA C-tail (GluRIIB^IIAtail). Experiments were repeated three times independently with similar results. B Schematic and representative images of boutons stained with anti-GluRIIA, -GluRIIA^tail, -GluRIIB, -pCaMKII, -CaMKII, and -DLG antibodies at NMJs of wild type and those containing only GluRB receptors in the indicated genotypes: G14 > GluRIIB (IIA/IIB^−/−) (w;G14-GAL4,GluRIIA^SP22/Df(2L)cl^h4;UAS-GluRIIB/+), G14 > GluRIIB^IIAtail (IIA/IIB^−/−) (w;G14-GAL4,GluRIIA^SP22/Df(2 L)cl^h4;UAS-GluRIIB^IIAtail/+). Experiments were repeated three times independently with similar results. Note that GluRB receptors containing the GluRIIA C-tail recruit pCaMKII at levels unchanged from the wild type. C Quantification of mean fluorescence intensity of the indicated antibodies in the indicated genotypes normalized to wild-type values (wild type: n = 13; GluRIIA^−/−, n = 11, p < 0.0001 for GluRIIA, p < 0.0001 for GluRIIA^tail, p < 0.0001 for GluRIIB, p < 0.0001 for pCaMKII, p = 0.9428 for CaMKII, p = 0.0012 for DLG; G14 > GluRIIB: n = 10, p < 0.0001 for GluRIIA, p < 0.0001 for GluRIIA^tail, p = 0.6436 for GluRIIB, p < 0.0001 for pCaMKII, p = 0.3599 for CaMKII, p = 0.0054 for DLG; G14 > GluRIIB^IIAtail: n = 11, p < 0.0001 for GluRIIA, p = 0.5391 for GluRIIA^tail, p = 0.6305 for GluRIIB, p = 0.0303 for pCaMKII, p = 0.9109 for CaMKII, p = 0.0468 for DLG). Repeated measures one-way ANOVA with Dunnett’s multiple comparisons test with a significance value of 0.05. p Value adjusted for multiple comparisons. Error bars indicate ±SEM. Asterisks indicate statistical significance using One-way ANOVA: *p < 0.05, **p < 0.01, ****p < 0.0001; ns, not significant. n values indicate biologically independent NMJs. Source data are provided as a Source Data file.