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. 2023 Feb 17;77(3):902–919. doi: 10.1002/hep.32612

FIGURE 7.

FIGURE 7

Mixed lineage kinase domain‐like pseudokinase (MLKL) inhibitor, GW806742X, impaired uptake of bioparticles in circulating monocytes from healthy controls (HC) and patients with alcohol‐associated hepatitis (AH). (A) Expression of messenger RNA (mRNA) for receptor interacting protein kinase (RIP) 1‐RIP3‐MLKL‐axis in peripheral blood mononuclear cells (PBMCs) isolated from HC and patients with AH. n = 5–9. (B) Human PBMCs were pretreated with or without GW806742X and then incubated with Escherichia coli bioparticles; n = 3. Mean fluorescent intensity (MFI) of bioparticles in CD14+ monocytes was assessed by flow cytometry, and MFI per cell was then calculated. (C) Engulfed bioparticles in CD14+/CD16+ monocytes isolated from human PBMCs were visualized by confocal microscopy. All images were obtained using a 63× objective (Zoom 4); n = 3. Values represent means ± SEM. Values with different superscripts are significantly different from each other within the same color bars; p < 0.05. hRIP1, human RIP1. hRIP3, human RIP3. hMLKL, human MLKL. DIC, differential interference contrast.