Table 2.

Antibody details and incubation protocols

Antibody	Isotype	Manufacturer	Incubation protocol
CD4	rabbit monoclonal	Abcam (Cambridge, UK)	HP, EDTA buffer, 1:50, o/n, RT
CD8	mouse monoclonal	Dako (Glostrup, Denmark)	HP, citrate buffer, 1:50, 1 h, RT
IL-1α	goat polyclonal	Santa Cruz (Dallas, USA)	1:50, o/n, 4 °C
IL-1ß	rabbit polyclonal	Abcam (Cambridge, UK)	1:100, o/n, 4 °C
TNF-α	mouse monoclonal	Santa Cruz (Dallas, USA)	1:100, o/n, 4 °C

HP Heat pretreatment, o/n overnight, RT Room temperature

Table 2 provides an overview of the antibodies used for immunohistochemical staining regarding isotype, manufacturer, and incubation protocols