Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 10;20:521. doi: 10.1186/s12951-022-01736-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 8 — In vivo immunogenic cell death induction and immunosuppressive TME reprogramming. Confocal microscopy images (A) and fluorescence intensity (B) of CRT protein exposure in tumor tissues treated with a) PBS; b) Fe-PHCN; c) Fe-PHCN@DOX; d) Fe-PHCN@DOX plus laser; and e) Fe-PHCN@DOX plus laser plus anti-PD-L1 nanobody. The quantification (C) and percentage (D) of matured DCs cells (CD11c⁺CD86⁺CD80⁺) by flow cytometric analyses after various treatments. Representative immunofluorescence images (E) and relative quantitative analysis (F) of hypoxia areas in tumors treated with different groups after staining with DAPI (blue) and anti-HIF-1α antibody (green). The quantification (G) and percentage (H) of M1-type macrophages (CD11b⁺F4/80⁺CD86⁺) by flow cytometric analyses after various treatments. The quantification (I) and percentage (J) of M2-type macrophages (CD11b⁺F4/80⁺CD206⁺) by flow cytometric analyses after various treatments. The quantification (K) and percentage (L) of CD8⁺ cytotoxic T (CD3⁺CD8⁺) cells by flow cytometric analyses after various treatments. M The percentage of CD4⁺ helper T (CD3⁺CD4⁺) cells by flow cytometric analyses after various treatments. N–P ELISA analysis of the levels of cytokines IL-6, TNF-⍺, and IFN-γ in serum of mice after various treatments. The scale bar represents 50 μm. The p values were analyzed using the Log-rank (Mantel-Cox) test. Data are presented as the mean ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001