Figure 2.

A) Phylogenetic tree of HgcA sequences front this study and B) fractional coverage of hgcA genes within predirted taxonomic groups. Unconfirmed methylators dominate hgcA sequence diversity in Lake Mendota, both numerically (A) and by coverage (B). For panel A, asterisks at the end of branches indicate sequence was binned, white all other branches are uncinned HgcA sequences from this study. Sequences were assigned a predicted taxonomic group based on phylogenetic clustery with HgcA reference sequences from NCBI and bin phytogenies of binned HgcA sequences (for delated tree with reference sequences, see Figure S3). Binned sequences outside of a monophyletic cluster are labeled with their bin name. In panel B, the back bars on the refer to the overall coverage of all hgcA sequences in each metagenome. Samples are arranged in order of increasing sulfide levels.