Table 5.
Extraction efficiency of nicotinamide adenine dinucleotide (NAD+) and nicotinamide mononucleotide (NMN) on DBS cards
| DBS card | Extraction efficiency (%) | |||||||
|---|---|---|---|---|---|---|---|---|
| NAD+ | NMN | |||||||
| Mean | SD | %CV | n | Mean | SD | %CV | n1) | |
| DMPK-A | 95.1 | 11.2 | 11.8 | 9 | 125.6 | 61.8 | 49.2 | 9 |
| DMPK-B | 90.9 | 11.4 | 12.5 | 14 | 87.7 | 36.9 | 42.1 | 14 |
| DMPK-C | 72.9 | 18.1 | 24.9 | 9 | 87.9 | 58.6 | 66.7 | 9 |
| 903 protein saver | 77.2 | 19.0 | 24.6 | 9 | 73.2 | 25.2 | 34.4 | 9 (1) |
Extraction efficiency (%) = concentration with cards (day 0) / concentration without cards (day 0) × 100
Lower limit of quantification: 0.5 to 2 μM (NAD+), 0.25 to 1 μM (NMN)
1)Numbers in parentheses represent the number of exclusions based on below the limit of quantification