Table 6.
Storage stability of nicotinamide adenine dinucleotide (NAD+) and nicotinamide mononucleotide (NMN) after extraction
| Storage stability in buffer (%) | ||||||
|---|---|---|---|---|---|---|
| NAD+ | NMN | |||||
| Mean | SD | %CV | Mean | SD | %CV | |
| Blood extraction solution (100% water) | ||||||
| DMPK-A | 145.8 | 12.5 | 8.6 | 73.7 | 15.6 | 21.1 |
| DMPK-B | 93.1 | 8.4 | 9.0 | 86.3 | 17.2 | 19.9 |
| DMPK-C | 200.6 | 62.0 | 30.9 | 63.7 | 19.3 | 30.2 |
| 903 protein saver | 266.3 | 59.6 | 22.4 | 78.4 | 17.1 | 21.8 |
| Blood extraction solution with acetonitrile (67% (v/v) acetonitrile water) | ||||||
| DMPK-A | 89.6 | 7.1 | 7.9 | 84.7 | 24.3 | 28.7 |
| DMPK-B | 100.0 | 16.1 | 16.1 | 84.1 | 33.7 | 40.0 |
| DMPK-C | 73.6 | 12.6 | 17.1 | 32.7 | 5.0 | 15.3 |
| 903 protein saver | 92.1 | 15.1 | 16.4 | 52.4 | 15.1 | 28.8 |
Storage stability in buffer (%) = concentration in blood extraction solution after storage for 71 days at − 20 °C / concentration before storage (day 0) × 100
The blood extraction solution was extracted from DBS cards after storage for 2 weeks at 4 °C