FIGURE 2.

MSI‐PCR and MMR‐IHC results in three discordant cases (Validation cohort). There were three discordant cases between MMR‐IHC and MSI‐PCR. (A), (B) Breast cancer (Case # 175, # 200), and (C) lung cancer (Case # 211). The left column shows the shift changes of the five microsatellite markers in the MSI‐PCR for each tumor and its corresponding buffy coat as a control. Right column shows the results of MMR‐IHC images. Original magnification ×100, scale bar 100 μm. In case # 175, there were three shifts (arrows) and four shifts in case # 200 and # 211 by MSI‐PCR. In these three cases, MMR‐IHC revealed no loss of nuclear staining of four proteins (MLH1, MSH2, MSH6, PMS2). All four MMR proteins showed positive nuclear staining in all cases (pMMR)