Table 1.
Gene and protein expression of TLRs and downstream molecules in studied population
| Target | Control (mean ± SD) (N = 45) | After 12 h (mean ± SD) (N = 45) | After 24 h (mean ± SD) (N = 45) | After 48 h (mean ± SD) (N = 45) | p value | ||
|---|---|---|---|---|---|---|---|
| Control vs. After 12 h | Control vs. After 24 h | Control vs. After 48 h | |||||
| Relative gene expression (Fold change) | |||||||
| Myd88 | 1.001 ± 0.08261 | 1.188 ± 0.5632 | 1.599 ± 0.4567 | 1.667 ± 0.6941 | NS | < 0.0001 | < 0.0001 |
| NFқB | 1.000 ± 0.07271 | 1.060 ± 0.5233 | 1.218 ± 0.6694 | 1.395 ± 0.4382 | NS | NS | < 0.0001 |
| TLR-3 | 1.000 ± 0.09613 | 1.220 ± 0.6738 | 1.371 ± 0.8069 | 1.746 ± 1.184 | NS | 0.0110 | 0.0004 |
| TLR-7 | 1.000 ± 0.1076 | 1.294 ± 0.8883 | 1.652 ± 1.055 | 1.860 ± 0.7372 | NS | 0.0005 | < 0.0001 |
| TLR-8 | 1.000 ± 0.06735 | 1.090 ± 0.2698 | 1.416 ± 0.8921 | 1.463 ± 0.7341 | NS | 0.0094 | 0.0004 |
| TLR-9 | 1.000 ± 0.1192 | 1.059 ± 0.3625 | 1.226 ± 0.5289 | 1.673 ± 1.075 | NS | 0.0226 | 0.0004 |
| Enzyme-linked immunosorbent assay (pg/ml) | |||||||
| IFN-Iα | 89.56 ± 34.96 | 102.2 ± 47.01 | 120.1 ± 52.12 | 140.1 ± 57.64 | NS | 0.0048 | < 0.0001 |
| IFN-Iβ | 95.31 ± 30.82 | 111.4 ± 38.57 | 132.7 ± 49.70 | 147.9 ± 52.39 | NS | 0.0002 | < 0.0001 |
| IL-1β | 64.91 ± 33.20 | 56.36 ± 28.35 | 47.02 ± 26.06 | 40.07 ± 24.50 | NS | 0.0167 | 0.0004 |
| TNF-α | 36.87 ± 19.50 | 30.44 ± 15.55 | 25.84 ± 12.09 | 22.71 ± 10.46 | NS | 0.0057 | 0.0002 |
| IL-8 | 121.8 ± 44.87 | 107.8 ± 43.71 | 94.51 ± 42.57 | 89.56 ± 38.08 | NS | 0.0118 | 0.0012 |
MyD88 Myeloid differentiation primary response 88, NFκB Nuclear factor kappa B, TLRs Toll-Like receptors, IFN-Is Type I interferons; ILs: Interleukins, TNF-α Tumor necrosis factor alpha. Data are presented as mean ± standard division (SD). P < 0.05 was considered as statistically significant