Table 2.
Filaria-reactive human IgE mAb target proteins
| IgE mAb |
Subject code |
ImmunoCAP reactivity (kUA/L) |
Binding to filarial worm extract |
EC50 (ng/mL) |
Mass spectrometry |
Antigen name |
Sequence ID | |
|---|---|---|---|---|---|---|---|---|
| ELISA | Immunoblot | |||||||
| 12D4 | P3 | 5.3 | + | +++ | 24.6 | WbSXP-1 | Antigen Wb14 | AF063940.1 |
| 4E1 | P3 | 0.5 | − | +++ | 144.6 | WbSXP-1 | Antigen Wb14 | AF063940.1 |
| 9C1 | P3 | 1 | + | +++ | 95.9 | TTR-76 | ? | VDM14676.1 |
| 12C2 | P3 | 0.3 | + | +++ | 95.9 | TTR-76 | ? | VDM14676 .1 |
| 5H1 | P1 | 1.4 | ++ | +++ | 6.5 | TTR-79 | ? | EJW78979 .1 |
| 18H7 | P1 | 0 | + | +++ | 43.3 | TTR-79, TTR-62 | ? | EJW86262.1, EJW78979.1 |
| 1A5 | P1 | 14.2 | ++ | +++ | ND | TTR-77 | ? | VDM13477.1 |
| 10D5 | P5 | 31 | ++ | − | ND | TTR-32 | ? | VDM07632.1 |
| 11G1 | P2 | 4.8 | + | − | ND | TTR-90 | ? | VDM14190.1 |
| 10H9 | P1 | 28.6 | ++ | − | ND | TTR-04 | ? | EJW80404.1 |
| 14B2 | P1 | 23.9 | + | +++ | ND | TTR-61 | ? | EJW84161.1 |
| 30A10 | P4 | 2.5 | + | − | ND | TTR-16 | ? | EJW86116.1 |
| 18D4 | P1 | 3.5 | + | − | ND | TTR-08 | ? | VDM13708.1 |
| 2E6 | P1 | 465.7 | +++ | +++ | 7.7 | Ladder protein, gp15/400 | Nematode polyprotein allergen-1 (NPA-1) | AAG31482.1 |
| 4E9 | P3 | 57.5 | +++ | − | 26.7 | Macrophage Migration Inhibitory Factor (MIF) | MIF−1 | EJW88743.1 |
| 11H12 | P1 | 12 | ++ | +++ | 1.7 | 24kDa secreted protein | Alt-1, P22 | VIO90327.1 |
The most prominent antigen(s) are listed for TTR-specific IgE mAbs (such as 5H1, 9C1 and 18H7) that cross-react with multiple members of the TTR family. ND: not determined due to low expression of target proteins.