Table 1.
Effect of dexamethasone on the percentage of CD45RA− memory T cell subpopulations after an O/N and 72 h-incubation with PBMCs previously treated O/N with and without IL-15.
| WO DEX O/N |
10−7 mol/L O/N |
10−6 mol/L O/N |
10−5 mol/L O/N |
|||||
|---|---|---|---|---|---|---|---|---|
| −IL15 | +IL15 | −IL15 | +IL15 | −IL15 | +IL15 | −IL15 | +IL15 | |
| O/N | ||||||||
| CD45RA− | ||||||||
| % CD45RA− | 25.2 | 18.6 | 30.1 | 21.6 | 30.8 | 21.4 | 33.0 | 28.1 |
| % CD3+ | 88.8 | 90.3 | 89.8 | 88.0 | 89.2 | 86.8 | 90.5 | 86.4 |
| % CD4+ | 76.8 | 79.2 | 75.8 | 81.3 | 77.5 | 82.6 | 78.6 | 84.1 |
| % CD8+ | 17.1 | 19.5 | 18.5 | 17.4 | 16.9 | 16.1 | 15.3 | 14.6 |
| CD45RA−CD4+ | ||||||||
| CD27+ (CM) | 80.4 | 80.5 | 80.9 | 77.7 | 80.4 | 78.4 | 78.0 | 74.7 |
| CD27− (EM) | 18.7 | 19.5 | 18.5 | 22.3 | 21.8 | 21.5 | 20.2 | 25.3 |
| CD127lowCD25+ (Treg) | 11.3 | 40.7*** | 9.8 | 42.0** | 8.2 | 40.1*** | 6.9 | 36.1*** |
| CD45RA−CD8+ | ||||||||
| CD27+ (CM) | 74.1 | 74.2 | 75.3 | 72.8 | 75.6 | 73.7 | 69.4 | 72.3 |
| CD27− (EM) | 24.8 | 25.8 | 23.8 | 27.2 | 23.8 | 26.3 | 24.2 | 27.7 |
| WO DEX 72 h |
10−7 mol/L 72 h |
10−6 mol/L 72 h |
10−5 mol/L 72 h |
|||||
| –IL15 | +IL15 | –IL15 | +IL15 | –IL15 | +IL15 | –IL15 | +IL15 | |
| 72-h incubation | ||||||||
| CD45RA− | ||||||||
| % CD45RA− | 19.1 | 20.1 | 21.8 | 22.8 | 23.5 | 23.4 | 23.5 | 25.3 |
| % CD3+ | 95.7 | 93.5 | 85.1 | 92.1 | 91.9 | 90.9 | 91.8 | 91.8 |
| % CD4+ | 88.7 | 77.5 | 84.0 | 79.7 | 85.2 | 78.5 | 84.5 | 80.2 |
| % CD8+ | 10.4 | 20.9 | 12.9 | 19.0 | 12.9 | 20.3 | 13.2 | 18.6 |
| CD45RA−CD4+ | ||||||||
| CD27+ (CM) | 71.0 | 75.7 | 73.0 | 76.9 | 76.9 | 82.0 | 76.9 | 81.2 |
| CD27− (EM) | 29.0 | 24.3 | 27.0 | 23.1 | 23.1 | 17.9 | 23.1 | 18.8 |
| CD127lowCD25+ (Treg) | 19.3 | 26.4 | 11.0 | 25.9*** | 6.4 | 27.9*** | 3.3 | 27.7*** |
| CD45RA−CD8+ | ||||||||
| CD27+ (CM) | 82.5 | 72.9 | 79.7 | 74.6 | 79.7 | 76.6 | 79.2 | 75.7 |
| CD27− (EM) | 17.5 | 26.9 | 20.3 | 25.4 | 20.3 | 23.4 | 20.8 | 24.3 |
The experiment has been designed following the scheme in supplemental Figure 1. Mean and standard error of the mean are shown. N = 3–4.
CM, central memory; EM, effector memory; IL-15, interleukin-15; O/N, overnight; PBMCs, peripheral blood mononuclear cells; Treg, regulatory T-cells.
**P ˂ 0.005.
***P ˂ 0.0001.