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. 2022 Nov 28;9:1008410. doi: 10.3389/fcvm.2022.1008410

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Copyright © 2022 Konrath, Mailer, Beerens, Englert, Frye, Kuta, Preston, Maas, Butler, Roest, de Laat and Renné.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thrombin generation (TG) in mouse whole blood assessed by CAT assay. (A) Left panel: real-time TG curves in 5 μl (left), 10 μl (middle) or 15 μl (right) recalcified mouse citrate whole blood. Right panel: corresponding endogenous thrombin potential (ETP) values. Data are presented as mean ± standard deviation (SD) of 10 replicates and coefficient of variation (CV) was calculated thereof. (B,C) TG measurements performed in mouse PPP (B) and whole blood (C) in the presence or absence of phospholipids (PL, 4 μM). PPP and whole blood activated with buffer only served as control. (D) A fixed volume of mouse PPP was supplemented with increasing concentrations of erythrocytes as indicated by hematocrit levels (HCT, 2.7–38.1 %). In all experiments shown, TG was stimulated with kaolin (10 μg/ml). Representative TG curves measured in triplicates of n = 3 individual experiments.