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. 2022 Nov 28;9:1008410. doi: 10.3389/fcvm.2022.1008410

Figure 5.

Figure 5

Contact-triggered TG is dependent on FXII and FXI levels in mouse whole blood. Venous whole blood collected from FXII-deficient (F12−/−) mice was stimulated with (A) kaolin (10 μg/ml), (B) ellagic acid (1 μg/ml), and (C) synthetic long-chain polyP (10 μg/ml, polymer size: ≥400 mers). (A–C) Real-time TG was assessed in recalcified blood following addition of human FXII (375 nM) or buffer. (D,E) Plasma-derived human FXII was added to whole blood collected from F12−/− mice at concentrations ranging from 0 to 750 nM. (F,G) Defined mixtures of whole blood from wild-type and FXI-deficient (F11−/−) mice resulted in FXI levels of 100–0%. (D–G) Kaolin (10 μg/ml) stimulated TG. Corresponding times to onset of TG (lag times) in (D,F) are shown in (E,G). Bars represent mean ± SD. Statistical significance was assessed by performing the Kruskal-Wallis test followed by Dunn's multiple comparison, with lag times compared to (E) 375 nM FXII or (G) 100% FXI. P > 0.05 (ns, not significant), P < 0.05 (*), P < 0.01 (**), P < 0.001 (***).