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. 2022 Nov 28;13:977809. doi: 10.3389/fimmu.2022.977809

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Copyright © 2022 del Rivero, Milberg, Bennett, Mitrani and Bellio

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AcAF Attenuates T Cell Proliferation 4 and 8 days after PHA Stimulation (A) Representative flow cytometry CD3+ gating. Representative histograms of proliferating CD3+ T cells in each group and graphed as percentages of the PHA vehicle control group following (B) 4-day PHA stimulation or (C) 8-day PHA stimulation. (D) Graphed percentages of PI- cells within CD3+ population. (Data graphed as mean ± SD; One way ANOVA with Tukey’s test; n=3 for each group; *=p<.05; **=p<.01; ***=p<.001; ns=not significant; Data is derived from 3 separate experiments. Each experiment used 1 independent PBMC donor treated with 1 of 3 acAF donors).