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. 2022 Nov 28;13:977809. doi: 10.3389/fimmu.2022.977809

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Copyright © 2022 del Rivero, Milberg, Bennett, Mitrani and Bellio

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AcAF Attenuates T Cell Activation 1 and 3 days after PHA Stimulation CD69+ and CD25+ cells were gated from CD3+ T cell population. Cells expressing CD69, CD25, and both CD69 and CD25 were analyzed within T cell population following (A) 1-day PHA stimulation and (B) 3-day PHA stimulation in each group and graphed as percentages of the PHA vehicle control group. (Data graphed as mean ± SD; One way ANOVA with Tukey’s test; n=3-4 replicates for each group; ns=not significant; *=p<.05; **=p<.01; ****=p<.0001; Data is derived from 3 separate experiments. Each experiment used 1 independent PBMC donor treated with 1 of 3 acAF donors).