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. 2022 Nov 28;13:977809. doi: 10.3389/fimmu.2022.977809

Figure 4.

Figure 4

Concentrated AF-EVs Have a Greater Effect on T Cell Proliferation and T Cell Activation than acAF (A) Representative histograms and proliferating CD3+ T cells in each group graphed as percentages of the PHA vehicle control group after 5 days of culture (B) CD69+ and CD25+ cells were gated from CD3+ T cell population. Percentages of cells expressing CD69, CD25, and both CD69 and CD25 were analyzed within T cell population in each group and graphed as percentages of the PHA vehicle control group after 3 days of culture. (Data graphed as mean ± SD; One way ANOVA with Tukey’s test; n=3-4 for each group; *=p<.05 **=p<.01; ***=p<.001; ****=p<.0001; Data is derived from 3 separate experiments. Each experiment used 1 independent PBMC donor treated with 1 of 2 acAF and 1 of 2 AF-EV donors).