a. Volcano plot of differentially expressed genes between H3.3K27M pons and H3.3K27M thalamus HGG. HOX genes are indicated in purple. Only genes with mean normalized expression > 100 are included.
b. Epigenomic state at NKX6-1 and PAX3 in representative H3.3K27M pons HGG primary tumors and cell lines. For scATACseq data, each track represents RPKM-normalized aggregated accessibility for one malignant single-cell population.
c. Co-expression of NKX6-1 and PAX3 in bulk RNAseq data for pons HGG with each K27M histone variant.