a. Expression of NKX6-1 and Pax3 in cell types of the normal developing mouse pons reference, showing their expression is largely mutually exclusive. The number of cells where both NKX6-1 and Pax3 are detected out of the total number of NKX6-1+ or Pax3+ cells of the cell type is indicated in parentheses.
b. Expression of NKX6-1 target genes with high cell type specificity in ependymal cells. Dendrogram represents cell clusters in the single-cell mouse pons reference, as in Figure S1.
c. Cell type-specificity score for inferred targets of NKX6-1 and Pax3 in the normal mouse pons. For a given gene, score represents the difference between the highest detection rate of the gene in any single-cell cluster in the normal mouse reference, and the detection rate of the gene in all other cells in the same sample (see Methods).