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. Author manuscript; available in PMC: 2023 Jun 5.
Published in final edited form as: Nat Genet. 2022 Dec 5;54(12):1865–1880. doi: 10.1038/s41588-022-01205-w

Figure 5. NKX6-1 is activated in H3.1K27M HGG.

Figure 5.

a. Immunohistochemistry staining of NKX6-1 in H3.1K27M HGG samples including patient tumors (N=5) and xenografts derived from the H3.1K27M patient-derived cell lines DIPGIV and DIPG36.

b. Immunohistochemistry for NKX6-1 protein in H3.3K27M HGG patient tumors (N=7).

c. Single-cell chromatin accessibility and RNA tracks for H3.1K27M pons HGG at the NKX6-1 locus. Each track represents RPKM-normalized aggregated accessibility/expression for one single-cell population. Normal cells and malignant cells are indicated. Asterisk (*) denotes tracks from scATAC data, all others are from scMultiome data. VISTA enhancer hs680 and NKX6-1 cis-regulatory module (CRMNkx6-1) are indicated. Schematic of CRMNkx6-1 indicates binding sites for SHH effector GLI transcription factors, SOX transcription factors, and homeodomain transcription factors, identified by Oosterveen et al, Developmental Cell, 201256.