(A) Fed serum adiponectin, (B) leptin and (C) corresponding leptin to adiponectin ratio for WT+HFD (n=8-12/group). (D) eWAT Lep in wild-type mice treated with vehicle or auranofin on HFD (n=4/group). RNA-Seq data shown as reads per kb million (RPKM). (E) The hypothalamus was stained and quantified for p-STAT3 (n=4/group) within the dorsal medial hypothalamus (DMH), ventral medial hypothalamus (VMH) and arcuate (ARC) nuclei of HFD mice treated with auranofin (1 mg/kg), scale=200 μm.
All data are mean ± SEM. *p<0.05 by unpaired t-test (A-D) and two-way ANOVA with Tukey’s multiple comparisons test (E)