Figure 1: Visual physiology in freely moving mice.

A) Schematic of recording preparation including 128-channel linear silicon probe for electrophysiological recording in V1 (yellow), miniature cameras for recording the mouse’s eye position (magenta) and visual scene (blue), and inertial measurement unit for measuring head orientation (green). B) Experimental design: controlled visual stimuli were first presented to the animal while head-fixed, then the same neurons were recorded under conditions of free movement. C) Sample data from a fifteen second period during free movement showing (from top) visual scene, horizontal and vertical eye position, head pitch and roll, and a raster plot of over 100 units. Note that the animal began moving at ~4 secs, accompanied by a shift in the dynamics of neural activity.