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. 2022 Nov 28;12:1064462. doi: 10.3389/fcimb.2022.1064462

Figure 8.

Figure 8

Succinate increases the protein expression of SUCNR1 and HIF-1a in mice. (A) Sucnr1 staining intensifies with an increasing of succinate concentration. Representative images of immunohistochemical staining for SUCNR1 in the control group and the NEC, Lsuc and Hsuc groups (n=3 per group). SUCNR1 localizes to the intestinal epithelium and lamina propria cells. Original magnification: ×100 and ×400; scale bar, 100 μm and 20 μm. (B–D) A representative western blot and graphs showing SUCNR1 and HIF-1a protein levels in intestine samples from four independent groups (n=7 per group and n=5 per group, respectively). The bars in the graph represent the means ± SD. Statistical significance was assessed with one-way ANOVA followed by Dunnett’s post hoc test. *P < 0.05.