FIGURE 6.

(a) Profile of normalized read counts (rpm) for total small RNA (sRNA) (length 16–46 nucleotides [nt]) produced in ∆ku70 and ∆Bcdcl1/∆Bcdcl2 mutants. (b) The numbers of unique sRNAs produced by Botrytis cinerea∆ku70 and ∆Bcdcl1/∆Bcdcl2 mutants with a length of 20–24 nt and their origin in the B. cinerea genome: ribosomal RNA (rRNA), transfer RNA (tRNA), transposable elements (TE), and other origins. The pie chart shows the results of three biological replicates. (c) The virulence of ∆Bcdcl1/∆Bcdcl2 compared with ∆ku70 and ∆ku70 with wild‐type B05.10 strain on tomato leaves. The diameter of lesions was measured at 3 days postinoculation (dpi), represented as a bar chart on the left side of panel (c). Error bars represent standard error of 72 inoculations from three independent experiments, and there were no significant differences between each comparison (statistical analyses were performed by Student's t test). Symptoms were photographed at 3 dpi, shown on the right side of panel (c)