Fig. 3.

Characterization of T-168658 inhibitory activity. a Dose-response inhibition effect of T-1686568 in three infected, immortalized cell lines. Data is averaged from four independent experiments; error bars are the SEM. b Viral titer from 48-h infected Calu-3 cells, with (100 μM, no host-cell toxicity observed) and without (DMSO) T-1686568 from 12 independent infections; error bars are the SEM. One-way ANOVA with Bonferroni multiple comparison correction, P < 0.0001. LOD – Limit of Detection. c Semi-dose-response inhibition effect of T-1686568 in primary donor-derived colon organoids, 72 hrs post-infection. Viral load interpolated to both non-infected cells (mock) control and an infected, untreated control, quantifying intracellular N protein. GSK3β inhibitor activity against the SARS-CoV-2 variants B.1.617.2 (delta) and BA1 (omicron). Immunofluorescence confocal images (d) of Huh-7.5.1 infected with B.1.617.2 with or without 10 μM T-1686568 treatment for 48 h. Host nuclei (blue), nucleocapsid (magenta), are shown, with scale bar (red) = 20 μm. e Plaque assays of B.1.617.2-infected Vero E6 cells, 2 days post-infection. f Plaque assays of BA.1-infected Vero E6 cells, 2 days post-infection