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Species-specificity assay of the PeOd360F/R primer using real-time polymerase chain reaction. The bacterial strains in lanes 1–29 are described in Table 1. Lane 30, distilled water (n = 3). (A) Amplification curves. (B) Melting curve. (C) Melting peak. The results of the melting peak analysis indicated that the amplified products have a melting temperature (Tm) of approximately 88°C. RFU, relative fluorescence units.
