Fig. 4.

TempO-Seq identified more DCA-induced DEGs than RNA-Seq in aged FFPE liver samples and showed better concordance with paired FROZ samples. Regression analysis of the intersection in log₂-transformed fold-change values in DEGs between paired TempO-Seq FFPE and TempO-Seq FROZ liver tissue samples induced by DCA at A. 427 mg/kg-d vs. vehicle control and B. 313 mg/kg-d vs. vehicle control. C. Total DCA-induced DEGs from paired TempO-Seq FROZ and FFPE tissue samples and overlap in DEGs relative to FROZ samples. DEGs were defined with these parameters: absolute value (fold change)> 2, FDR-adjusted p-value< 0.05. Percent DEG overlap calculated as DEG Overlap/(FFPE + FROZ DEGs)* 100. Percent FFPE DEG in FROZ calculated as (DEG Overlap/FROZ DEGs)* 100. Abbreviations. DEG-differentially expressed genes, DCA- dichloroacetic acid, FFPE-formalin-fixed paraffin-embedded, FROZ-frozen, RNA-Seq- RNA-Sequencing, FDR- false discovery rate.