Fig. 6.

DCA-induced DEGs detected by TempO-Seq in FFPE liver tissue more consistent with DEGs detected by RNA-Seq of FROZ liver tissue than RNA-Seq of FFPE. Regression analysis of the intersection in log₂ transformed fold-change values in DEGs between TempO-Seq FFPE and RNA-Seq FROZ liver tissue samples induced by DCA at A. 427 mg/kg-d vs. vehicle control and B. 313 mg/kg-d. vs. vehicle control C. Total DCA-induced DEGs from TempO-Seq FFPE and RNA-Seq FROZ liver tissue samples as well as overlap in DEGs relative to RNA-Seq FROZ. DEGs were defined with these parameters: absolute value (fold change)> 2, FDR-adjusted p-value< 0.05. DEG overlap includes DEGs in common between RNA-Seq and TempO-seq. Percent DEG overlap calculated as DEG Overlap/(RNA-Seq + TempO-seq DEGs)* 100. Percent TempO-seq DEG in RNA-Seq calculated as (DEG Overlap/RNA-Seq DEGs)* 100. Abbreviations. DEG-differentially expressed genes, DCA-dichloroacetic acid, FFPE-formalin-fixed paraffin-embedded, FROZ-frozen, RNA-Seq- RNA-Sequencing, FDR- false discovery rate.