Table 5.
Summary of Ames assay positive controls.
|
S. typhimurium strain |
S9 Activation |
Positive Control |
Solvent | Concentration [µg/plate] |
|---|---|---|---|---|
| TA98 | No activation | 2-nitrofluorene | DMSO | 4.0 |
| TA98 | Rat liver | 2-aminoanthracene | DMSO | 2.0 |
| TA98 | Rat liver | Benzo[a]pyrene | Acetone | 5 |
| TA100 | No activation | Sodium azide | Type I Water | 1.0 |
| TA100 | Rat liver | 2-aminoanthracene | DMSO | 2.0 |
| TA102 | No activation | Mitomycin C | Type I Water | 0.5 |
| TA102 | Rat liver | 2-aminoanthracene | DMSO | 7.5 |
| TA1535 | No activation | Sodium azide | Type I Water | 1.0 |
| TA1535 | Rat liver | 2-aminoanthracene | DMSO | 4.0 |
| TA1537 | No activation | 9-aminoacridine | DMSO | 100.0 |
| TA1537 | Rat liver | 2-aminoanthracene | DMSO | 4.0 |
For the 1R6F reference cigarette, TPM extract dose levels of 0, 25, 50, 75, 100, 125, 250, 500 µg TPM/plate were tested. For each smokeless test item in CAS dose levels of 0, 0.71, 1.43, 2.86, 5.71, 8.57, 11.43, 14.29, 17.14, 22.86, 28.57 mg SP/plate were tested for replicate 1 and dose levels of 0, 0.71, 1.43, 2.86, 5.71, 11.43, 14.29, 28.57 mg SP/plate were tested for replicate 2 and 3.
The assays were completed in triplicate.