Table 7.

Summary of results from the MTT viability assay for 1R6F and smokeless products extracts in the EpiGingival™ 3D Tissue Model (4-h and extended overnight Treatment). Values represent average viability calculated from 3 wells per tissue at a given concentration, (duplicate tissues were tested).

Values represent average viability calculated from 3 wells per tissue at a given concentration, (duplicate tissues were tested)
Product	Dose Level (%, v/v) or (%,w/v) SP	Average Viability (%) 4-h Treatment (Initial Irritation assay)	Average Viability (%) 4-h Treatment (Confirmatory irritation assay)	Average Viability (%) 18-h Treatment (Initial Irritation assay)	Average Viability (%) 18-h Treatment (Confirmatory irritation assay)
1R6F	0.625	102	97	108	113
	1.25	135	95	103	112
	2.50	101	91	99	110
	5.00	98	100	100	111
VC	100 µL	100	100	100	100
SLS	0.5 or 0.15	24	28	6	18
NP1 M 6 mg	3.75	97	98	103	99
	7.5	92	102	107	93
	15	97	93	97	126
	30	101	98	86	76
VC	100 µL	100	100	100	100
SLS	0.5 or 0.15	15	31	21	14
NP1 M 9 mg	3.75	101	91	106	93
	7.5	98	88	107	83
	15	100	91	95	83
	30	102	86	88	92
VC	100 µL	100	100	100	100
SLS	0.5 or 0.15	29	22	8.4	5
NP1 SM 11.2 mg	3.75	97	105	95	97
	7.5	102	94	95	104
	15	97	91	93	81
	30	81	81	79	75
VC	100 µL	100	100	100	100
SLS	0.5 or 0.15	20	27	12	9

VC = 5 % Ethanol in Phosphate Buffered Saline (1R6F only) or VC = Vehicle Control; Complete Artificial Saliva

SLS - Sodium Lauryl Sulfate (%, w/v).