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. 2022 Jul 12;9:1528–1536. doi: 10.1016/j.toxrep.2022.07.007

Fig. 3.

Fig. 3

A: The presence of ver-1gene PCR products. Lane 1, 2: Aspergillus parasiticus, Lanes 3, 4: Aspergillus flavus; M: 100 bp ladder. B: The presence of afl-R gene PCR products. Lane 1, 2: Aspergillus parasiticus, Lanes 3, 4 and 5: Aspergillus flavus; M: 100 bp ladder. C: Chromatogram of standard working toxins used to prepare calibration diagrams Based on RT [Retention Time], the retention time was 7.41 for Afla G2, 8.49 for Afla G1, 9.78 for AflaB2, and 11.35 for AflaB1. D: Chromatogram of Aspergillus flavus strains capable of producing all four forms of aflatoxins [B1, B2, G1, G2]. Aflatoxin G2 took 7.31 min to withdraw, Aflatoxin G1 took 8.36 min, Aflatoxin B2 took 9.63 min, and aflatoxin B1 took 11.18 min. As can be observed in the image, aflatoxin B1 has a larger concentration than the others, indicating that this toxin is the first result of aflatoxins biosynthesis, with other forms of aflatoxins formed subsequently.