Table 1.
The sequence of primers used.
| Gene | Primer sequence (5′−3′) | Sequence amplified | Product size (bp) |
|---|---|---|---|
| KL | F_GAATACGCAAAGTAGCCACAAAG R_GAGCAAGACTCACTGAGGATG |
XM_039089809.1 | 104 |
| FSHR | F_ATCATTTGCTGGATTTGGAGAC R_TTGGGTAGGTTGGAGAACACAT |
NM_199237.2 | 96 |
| GDF-9 | F_CAATACCGTCCGGCTCTTCAG R_AGGGGTCCTGTCATCTGGTTG |
XM_017597502.2 | 72 |
| FIGLA | F_CAGGAAGCCCAGTAAAGTTGA R_CTTCTTTCTTCAAGCCACTTGC |
XM_575589.7 | 218 |
| BCL-2 | F_CGGGAGAACAGGGTATGA R_CAGGCTGGAAGGAGAAGA |
NM_016993.2 | 149 |
| BAX | F_CAGGACGCATCCACCAAGAAG R_TGCCACACGGAAGAAGACCTC |
NM_017059.2 | 135 |
| B-Actin | F_ CCCATCTATGAGGGTTACGC R_TTTAATGTCACGCACGATTTC |
NM_031144.3 | 150 |
bp: base pair, Kl: Rattus norvegicus Klotho, FSHR: follicle-stimulating hormone receptor, GDF-9: growth differentiation factor-9, FIGLA: folliculogenesis specific BHLH transcription factor, BCL-2: B-cell lymphoma 2, BAX: Bcl-2 Associated X-protein 4, B-Actin