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. 2022 Nov 28;13:1057413. doi: 10.3389/fphys.2022.1057413

FIGURE 9.

FIGURE 9

The N- and C-termini of Beat localize to different subcellular compartments. (A–C''') Confocal images of dissected third instar larvae expressing GFP-Beat-Cherry in muscles or motor neurons. Native GFP (green), native Cherry (magenta), DAPI (blue). (A–A''') Under control of Mef2-Gal4, N-terminal GFP distributes over the entire muscle surface, while C-terminal Cherry is predominantly nuclear (arrows). (B–B''') Ventral nerve cord (dorsal view) showing expression of GFP-Beat-Cherry in motor neurons using FasII-Gal4. (C–C''') White frame in (B''') enlarged. Arrows label a single RP motor neuron, showing punctate GFP in the cytoplasm that does not overlap with nuclear Cherry. (D–D''') Confocal images of transiently transfected S2 cells expressing GFP-Beat-Cherry. While N-terminal GFP accumulates predominantly in the cytoplasm (D), C-terminal Cherry (D') co-localizes with nuclear DAPI (D''). Merged images (D'''). (E-E''') Expression of GFP-Beat-Cherry under control of FasII-Gal4 in stage 15 embryos (ventral view). White frame in (E'') is enlarged in (E'''). GFP and Cherry separate already when motor axons actively migrate into the periphery.Scale bars: 5, 10, 20 and 100 μm as indicated.