Fig. 1.

Translaminar single unit recording in awake mouse auditory cortex. (A) Mice were head fixed atop a spherical treadmill. (B) Translaminar probes recorded neuronal activity across cortical layers. (C) Auditory cortical depth estimation. (1.) The putative span of cortex was estimated based on the span of channels with multiunit responses to search stimuli. Single units were then classified as Superficial, Middle, or Deep according to their relative depth within this span. (2–4) Magnified view of example responsive channels. (D) Classification of single units as narrow (putative inhibitory) or broad spiking (predominantly excitatory). Left: Example spike waveform (median ± MAD). Right: Narrow and broad spiking units clustered by trough-peak delay and FR.