FIG. 3.

Hybridization analysis of B. turicatae OZ-1 DNA fractionated by 2D-CHEF-PFGE. Agarose plugs containing B. turicatae DNA were prepared, and 2D-CHEF-PFGE was conducted as described in the text. The direction of migration in each dimension is indicated with arrows, and the migration position of the size standards are shown. The C designation above the size standards indicates the migration position of the 910-kb linear chromosome. Note that under the electrophoresis conditions employed, the 220-kb linear plasmid migrates just ahead of the chromosome. Panel A shows the ethidium bromide-stained gel. The blots in panels B and C were probed with the bdrAsf1R1 and bdrBsfR1 oligonucleotides, respectively.