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. 2022 Dec 12;13:7675. doi: 10.1038/s41467-022-35225-1

Fig. 4. Virus titres of SARS-CoV-2-infected mice via diverse injection routes.

Fig. 4

a An anatomical graphic depicting the murine brain, eye (optic nerve), trigeminal nerve and lung. Created with BioRender.com. b Illustration showing the diverse injection routes: intranasal (IN), intratracheal (IT), intracerebral (IC), eye-drop (ED) and intravenous (IV) routes. Created with BioRender.com. c K18-hACE2 mice were inoculated with 104 PFU SARS-CoV-2 via five different injection routes (n = 8 per injection route; n = 4 for 3 dpi and 6 dpi, respectively). Viral RNA levels in the lungs, brain, eye globes, trigeminal nerve and optic nerve were analysed at 3 and 6 dpi using RT-qPCR (3 dpi, blue; 6 dpi, red). Viral RNA copies were subjected to cut-off at 103 copies/μg. Symbols represent means ± SEM. SARS-CoV-2 severe acute respiratory syndrome coronavirus 2, PFU plaque-forming unit; dpi: days post-infection.