Figure 2.

Monocyte-derived alveolar macrophages (moAMs) express HB-EGF. PrimeFlow RNA assays show the presence of HB-EGF ⁺ alveolar macrophages (AMs) (CD11c ⁺ SiglecF^hi), HB-EGF ⁺ moAMs (CD11c ⁺ CD11b ⁺ SiglecF^int), and HB-EGF ⁺ interstitial macrophages (IMs) (CD11b ⁺ SiglecF^lo) in C57Bl/6J mice in control animals (A). Oral-pharyngeal administration of bleomycin induces an expansion of HB-EGF ⁺ moAMs and IMs after 21 days (B) compared with mice treated with a saline control (A) in C57Bl/6J mice. t-Distributed Stochastic Neighbor Embedding (tSNE) data is representative of one experiment with three mice per group. All data representative of 3 experiments, n = 3.