Table 3.
Identification of MGO-polyphenol adducts by UPLC-qTOF-MS.
| RT (min) | Molecular formula | Polarity | Theoretical mass | Observed mass | Error (ppm) | MS/MS fragments | Rooibos + MGO | |
|---|---|---|---|---|---|---|---|---|
| Orientin-MGO adduct-1 | 13.82 | C24H24O13 | Positive | 521.1295 | 521.1292 | −0.57 | 503, 485, 467, 449, 437, 407, 383,355, 337, 325 | + |
| Orientin-MGO adduct-2 | 15.99 | C24H24O13 | Positive | 521.1295 | 521.1293 | −0.38 | 503, 485, 467, 449, 437, 407, 383,355, 337, 325 | + |
| Isoorientin-MGO adduct-1 | 14.00 | C24H24O13 | Positive | 521.1295 | 521.1292 | −0.57 | 503, 485, 467, 449, 437, 407, 383,355, 337, 325 | + |
| Isoorientin-MGO adduct-2 | 17.09 | C24H24O13 | Positive | 521.1295 | 521.1293 | −0.38 | 503, 485, 467, 449, 437, 407, 383,355, 337, 325 | + |
| Vitexin-MGO adduct-1 | 18.92 | C24H24O12 | Positive | 505.1346 | 505.1342 | −0.79 | 487, 469, 451, 433, 421, 391, 367, 399, 321, 309 | tr |
| Vitexin-MGO adduct-2 | 20.93 | C24H24O12 | Positive | 505.1346 | 505.1340 | −1.18 | 487, 469, 451, 433, 421, 391, 367, 399, 321, 309 | tr |
| Isovitexin-MGO adduct-1 | 20.32 | C24H24O12 | Positive | 505.1346 | 505.1344 | −0.40 | 487, 469, 451, 433, 421, 391, 367, 399, 321, 309 | tr |
| Isovitexin-MGO adduct-2 | 24.60 | C24H24O12 | Positive | 505.1346 | 505.1345 | −0.20 | 487, 469, 451, 433, 421, 391, 367, 399, 321, 309 | tr |
| Aspalathin-MGO adduct | 15.83 | C24H28O13 | Negative | 523.1452 | 523.1452 | 0.00 | 505, 451, 433, 403, 385, 361, 331, 263, 235, 221, 209, 167 | + |
“+”, detected; “tr”, trace amount.