Table 1.
Strains and plasmids used in this study
| Characteristics | Source | |
|---|---|---|
| Strains | ||
| E. coli BL21 (DE3) | F− ompT hsdSB (rB− mB−) gal (λ cI857 ind1 sam7 nin5 lacI lacUV5-T7 gene1), dcm (DE3) | Transgen |
| E. coli Trans1-T1 | F− ϕ80 (lacZ) ΔM15 ΔlacX 74 hsdR(rk−mk−) ΔrecA 1398endA1tonA | Transgen |
| E. coli BL4 | BL21(DE3) carrying pE-4CL4 | Laboratory |
| E. coli BL5 | BL21(DE3) carrying pE-4CL5 | Laboratory |
| E. coli BLS5 | BL21(DE3) carrying pR-PpSTS-Ptr4CL5 | This study |
| E. coli BLPT | BL21(DE3) carrying pR-PtSTS-Ptr4CL5 | This study |
| E. coli BLPS | BL21(DE3) carrying pR-PsSTS2Q361R-Ptr4CL5 | This study |
| E. coli BR4S | BL21(DE3) carrying pR-Ptr4CL4-PpSTS | This study |
| E. coli BRS4 | BL21(DE3) carrying pR-PpSTS-Ptr4CL4 | This study |
| E. coli BE4S | BL21(DE3) carrying pE-Ptr4CL4-PpSTS | This study |
| E. coli BES4 | BL21(DE3) carrying pE-PpSTS-Ptr4CL4 | This study |
| E. coli BC4S | BL21(DE3) carrying pC-Ptr4CL4-PpSTS | This study |
| E. coli BCS4 | BL21(DE3) carrying pC-PpSTS-Ptr4CL4 | This study |
| E. coli BRT4S | BL21(DE3) carrying pRT-Ptr4CL4-PpSTS | This study |
| E. coli BRTS4 | BL21(DE3) carrying pRT-PpSTS-Ptr4CL4 | This study |
| E. coli BET4S | BL21(DE3) carrying pET-Ptr4CL4-PpSTS | This study |
| E. coli BETS4 | BL21(DE3) carrying pET-PpSTS-Ptr4CL4 | This study |
| E. coli BCT4S | BL21(DE3) carrying pCT-Ptr4CL4-PpSTS | This study |
| E. coli BCTS4 | BL21(DE3) carrying pCT-PpSTS-Ptr4CL4 | This study |
| Plasmids | ||
| pRSFDuet-1 | RSF ori with PT7; Kanr | Novagen |
| pCDFDuet-1 | CDF ori with PT7; Smr | Novagen |
| pETDuet-1 | PBR322 ori with PT7; Ampr | Novagen |
| pE-4CL4 | pETDuet-1 with Ptr4CL4 | Laboratory |
| pE-4CL5 | pETDuet-1 with Ptr4CL5 | Laboratory |
| pR-PpSTS | pRSFDuet-1 with PpSTS | Generay Biotech |
| pR-PtSTS | pRSFDuet-1 with PtSTS | Generay Biotech |
| pR-PsSTS2Q361R | pRSFDuet-1 with PsSTS2Q361R | Generay Biotech |
| pR-PpSTS-Ptr4CL5 | pRSFDuet-1 with PT7-PpSTS and PT7-Ptr4CL5 | This study |
| pR-PtSTS-Ptr4CL5 | pRSFDuet-1 with PT7-PtSTS and PT7-Ptr4CL5 | This study |
| pR-PsSTS2Q361R-Ptr4CL5 | pRSFDuet-1 with PT7-PsSTS2Q361R and PT7-Ptr4CL5 | This study |
| pR-PpSTS-Ptr4CL4 | pRSFDuet-1 with PT7-PpSTS and PT7-Ptr4CL4 | This study |
| pR-Ptr4CL4-PpSTS | pRSFDuet-1 with PT7-Ptr4CL4 and PT7-PpSTS | This study |
| pE-PpSTS-Ptr4CL4 | pETDuet-1 with PT7-PpSTS and PT7-Ptr4CL4 | This study |
| pE-Ptr4CL4-PpSTS | pETDuet-1 with PT7-Ptr4CL4 and PT7-PpSTS | This study |
| pC-PpSTS-Ptr4CL4 | pCDFDuet-1 with PT7-PpSTS and PT7-Ptr4CL4 | This study |
| pC-Ptr4CL4-PpSTS | pCDFDuet-1 with PT7-Ptr4CL4 and PT7-PpSTS | This study |
| pRT-PpSTS-Ptr4CL4 | pRSFDuet-1 with PT7-PpSTS-Ptr4CL4 | This study |
| pRT-Ptr4CL4-PpSTS | pRSFDuet-1 with PT7 -Ptr4CL4-PpSTS | This study |
| pET-PpSTS-Ptr4CL4 | pETDuet-1 with PT7-PpSTS-Ptr4CL4 | This study |
| pET-Ptr4CL4-PpSTS | pETDuet-1 with PT7 -Ptr4CL4-PpSTS | This study |
| pCT-PpSTS-Ptr4CL4 | pCDFDuet-1 with PT7-PpSTS-Ptr4CL4 | This study |
| pCT-Ptr4CL4-PpSTS | pCDFDuet-1 with PT7-Ptr4CL4-PpSTS | This study |