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. 2022 Dec 12;18:134. doi: 10.1186/s13007-022-00963-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The spectrum of coloured agar under various lighting conditions. A The range of colours tested for contrast with nematodes and filtration potential of the background. B The colour red agar under four different lighting conditions. From left to right: White, Red, Green and Blue light. The blue lighting condition with the red agar were the most ideal combination for quantifying nematodes using the segmentation tool. The bottom image row has the contrast and brightness adjusted for visibility. Examples of female nematodes are highlighted with yellow arrows