Table 2.
Monoclonal antibodies used for immunolabelling and combinations (panels) used to phenotype leukocytes
| Abbreviation | Clone | Specificity | Fluorochrome | Panel | ||
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| CD41/61−Fitc | 11C3a | Chicken CD41/61 integrin (GPIIb-IIIa) | Fluoresceinc | X | – | – |
| KUL01-RPE | KUL01b | Chicken mannose receptor MRC1L-B [36] | R-phycoerythrinc | X | – | – |
| MHCII−PerCp/Cy5.5 | 2G11b | Chicken MHC class II | Peridinin chlorophyll-cyanine 5.5d | X | – | – |
| CD45−PE/Cy7 | UM16-6a | Chicken CD45, all isoforms [37] | R-phycoerythrin-cyanine 7d | X | – | – |
| Bu1-APC/Cy7 | AV20b | Chicken Bu-1 (chB6) transmembrane protein | Allophycocyanin-cyanine 7d | X | – | – |
| CD4−Fitc | CT-4b | Chicken CD4 | Fluoresceinc | – | X | – |
| CD8−RPE | EP42b | β-chain of chicken CD8 | R-phycoerythrinc | – | X | X |
| TCRγ/δ−PerCp/Cy5.5 | TCR-1b | Chicken γ/δ T-cell receptor | Peridinin chlorophyll-cyanine 5.5d | – | X | – |
| CD8α−Cy5 | 3-298b | α-chain of chicken CD8 | Cyanine 5c | – | X | X |
| CD25−PE/Cy7 | AV142a | Chicken CD25, interleukin-2 receptor α-chain | R-phycoerythrin-cyanine 7c | – | X | – |
| TCRγ/δ−Fitc | TCR-1b | Chicken γ/δ T-cell receptor | Fluoresceinc | – | – | X |
| CD4−PACBLU | CT-4b | Chicken CD4 | Pacific Blue™c | – | – | X |
Panels 1 and 2 were used for whole blood samples, panel 3 was used for activated T-cells in culture.
X: used in panel; –: not used in panel.
aPurchased from Bio-Rad Antibodies.
bPurchased from SouthernBiotech.
cFluorochrome conjugated by manufacturer.
dFluorochrome conjugated using Lightning-Link® conjugation kits (abcam) according to the manufacturer’s protocol.