Table 2.
Primers used in this study
| Name | Sequence (5′–3′) |
|---|---|
| Primers for construction of mutants | |
| ∆rpoN-S-F | GCTCTAGACAACCTGATGGCGGTACTG |
| ∆rpoN-S-R | CCTGTTGTCT GCAACCTCGGTATCCGTACT |
| ∆rpoN-X-F | CCGAGGTTGC AGACAACAGGATGAGGAAGACG |
| ∆rpoN-X-R | GGGGTACCCTGCCCATTTTTTCGCG |
| ∆rpoN-F | CAACCTGATGGCGGTACTG |
| ∆rpoN-R | CTGCCCATTTTTTCGCG |
| ∆T2SS-2-S-F | GCTCTAGAGTTAGTGAGTCGTCTATGTTGGCTT |
| ∆T2SS-2-S-R | GCTACGCCATCGTCACCCCGCCGTTAT |
| ∆T2SS-2-X-F | CGGGGTGACGATGGCGTAGCCGGTGGT |
| ∆T2SS-2-X-R | GGGGTACCCACCACGCCGCCTTGT |
| ∆T2SS-2-F | GTTAGTGAGTCGTCTATGTTGGCTT |
| ∆T2SS-2-R | CACCACGCCGCCTTGT |
| Primers for identification of plasmid | |
| pRE112-U-F | CACTGTTCGTCCATTTCCG |
| pRE112-D-R | TTCGTCTCAGCCAATCCCT |
| pBAD33-U-F | AACAAAGCGGGACCAAAG |
| pBAD33-D-R | AGAGCGTTCACCGACAAA |
| pMS402-U-F | GGTCAAATGAATGCAGGGCT |
| pMS402-D-R | AGAGTCATTCAATATTGGCAGGT |
| Primers for construction of complemented strain | |
| pBAD33-rpoN+-F | GGGGTACCATGAAGCCAAGTTTACAACTCAAG |
| pBAD33-rpoN+-R | GCTCTAGATTAAACCAAGCGTTTACGCTG |
| Primers for lux | |
| lux-flaC-F | CCGCTCGAGATTTCTTGACATGCCGCGTT |
| lux-flaC-R | CGGGATCCATCTCCGTTAAACTTGCCGC |
| lux-motA-F | CCGCTCGAGTCCCCAGGTCTCAAAATCGT |
| lux-motA-R | CGGGATCCCATCAAACCTCTGTGCTCGT |
| lux-fliAL-F | CCGCTCGAGGCAAGCTGGCATCTCTGTAC |
| lux-fliAL-R | CGGGATCCCTATCCTCTGTCTACCGCGC |
| Primers for qRT-PCR | |
| gyrB-RT-F | GATTTGCGCACTGGGTAGCC |
| gyrB-RT-R | GCGGCTGTTTGGATCCATGG |
| flaC-RT-F | TTGCGATCGACTCATCCCTG |
| flaC-RT-R | CAGGATCTGCTGCTTGGTCA |
| motA-RT-F | GGTGGTTTTTTTCGCAGC |
| motA-RT-R | TTCCTCTTACAAATAAAACGCC |
| fliAL-RT-F | ATAGGTTTTGCCTTGCGATA |
| fliAL-RT-R | TCTATCCTCTGTCTACCGCG |
| tssB-RT-F | GTGGTCAGCAGGCCGAAATC |
| tssB-RT-R | TTGGCACCCCTCTTCCAGAC |
| tssC-RT-F | GGGTATTGCTGCGTTGGTGG |
| tssC-RT-R | TGGTGGCATGCAAGACTTGG |
| tssE-RT-F | TGTCATTTCAAGTCACAGCACA |
| tssE-RT-R | GGTCACCTGCTCGATATTCTCCA |
| tssF-RT-F | TTGTACGCGGCGATGAAACG |
| tssF-RT-R | CAAATGACGGTGAGCTGCCC |
| tssG-RT-F | CAATCGCGCCAAGATGCTGT |
| tssG-RT-R | ACCTTATCGCCCAGCACCAA |
| tagH-RT-F | ATCGTGAGCTGGCCAGTCAT |
| tagH-RT-R | GGCTGGGATGCTGTAGGGAT |
| tssJ-RT-F | ATGTGGCGATGAACCCGGAT |
| tssJ-RT-R | CGTAATCGGTGGCCAACAGG |
| tssK-RT-F | TGCTCGCTTAAGTGCCCTGA |
| tssK-RT-R | GACCTCACTGACGCCACTGA |
| tssL-RT-F | ACCATGCCGCACAGCTAGAT |
| tssL-RT-R | TCTGTCACCACGCGCTGATA |
| tssH-RT-F | CGAAAGCAAAGCAAACGCCG |
| tssH-RT-R | CTTCGCCAACCACAATCGGG |
| vasH-RT-F | ATTCACGACCGCCTGACTGT |
| vasH-RT-R | GTGATCCAGTGTGCGCTTCG |
| tagO-RT-F | AGGCTCCTGTGTCGGTTAGC |
| tagO-RT-R | CCAAATGCGGGTAATGGCGT |
| tssA-RT-F | TGCGCGAGATGACCTTCAGT |
| tssA-RT-R | ACTCGCTGCCTAAATCCGCT |
| tssM-RT-F | CAGTTTGAGTGCGGCGGATC |
| tssM-RT-R | TCATTGCCAATCACCGCTGC |
| impA-RT-F | CATTGCAGGGTATGGAGCGC |
| impA-RT-R | CAAAGGCGAGGCTTGCTCAG |
| paaR-RT-F | ATTACGGCCAGCCCTGATGT |
| paaR-RT-R | GCTTATCGTGCGGCTCAAGG |
| vgrG1-RT-F | GGGCATGCGGTGATTGTGTT |
| vgrG1-RT-R | CCTGCCCAGCCTGATCTTCA |
| hcp-RT-F | TCACCTCTGAATCCGTGGGC |
| hcp-RT-R | GCGCAACGGTGAACTGGAAT |
| vgrG2-RT-F | CCGGGTTGTGCGTTCAGTTT |
| vgrG2-RT-R | CACGGTAGCGATTTGTGGGC |
| orf00078-RT-F | TACGCCTGAGTGATGCCCAG |
| orf00078-RT-R | TCCTCACTTCCCCACCATGC |
| orf00080-RT-F | TCGGCCAGGCGATACAACTT |
| orf00080-RT-R | CGGATCTCACGCCACTCAGT |
| yghF2-RT-F | CAAGGATAACGTGCTGGTCG |
| yghF2-RT-R | AATCCGGTAATTCACGCAGC |
| gspD2-RT-F | CGCCAGATATCATGCAGTCG |
| gspD2-RT-R | GCACGCCGAAATTGATGTTG |
| gspE2-RT-F | GCCTTACTCACTAGCGCCTA |
| gspE2-RT-R | TGGCGTTGATGAGCTTGATG |
| gspF2-RT-F | TAGCCGAGCAGTGTGAGAAA |
| gspF2-RT-R | TGGCGCAAAACAACTCATCA |
| gspG2-RT-F | AGTGATGGTGGTGATCGTGA |
| gspG2-RT-R | TCGGGTACACGCTGTTATCC |
| gspH2-RT-F | CATTAGCTGGTCGACAAGCC |
| gspH2-RT-R | TACTGTCGAGCCACACTTGT |
| gspI2-RT-F | GTATTGGCGCAGTAAGTCGG |
| gspI2-RT-R | TCATAGCGCTGACTCCGTAC |
| gspJ2-RT-F | TCAGGTGGTTGATGGGGTAC |
| gspJ2-RT-R | ATTGTAAAAGTCCGTCGCCG |
| gspK2-RT-F | TTAGATGGGCAAGTCACCGT |
| gspK2-RT-R | CGAACTGATGGCGTTGAACA |
| gspL2-RT-F | TTTAGACGTGGCCCAGGATT |
| gspL2-RT-R | ACATCGGGTTTTGTTTCGGG |
| gspM2-RT-F | TGTTGGTTGGCGGATTGTAC |
| gspM2-RT-R | CAATTGCCGTAACAGCTGCT |
| spaS-RT-F | CAACCACACCAGTAGACGCA |
| spaS-RT-R | AACGGATGTGAAATTGGCGC |
| spaR-RT-F | TTCGCTGGATCAATCGTGCT |
| spaR-RT-R | GTCATGCTGGGCCTGTTACT |
| spaQ-RT-F | GTTTCACCATACCAGCCCGA |
| spaQ-RT-R | ATGATTTGATGTTTGCGGGCAA |
| spaO-RT-F | GCCAACCACGTGACAACATC |
| spaO-RT-R | GTGACCGTTGATTTGCCACC |
| spaN-RT-F | GCTGCTGATGTGGATTTCGC |
| spaN-RT-R | CAGCCTTCAGATTCGGTGGT |
| invC-RT-F | AGCTATCAAACACCGACGCA |
| invC-RT-R | TTCGCGATCAGGGTAAACGG |
| invG-RT-F | CGCCCATCCAGAGTGCTTAT |
| invG-RT-R | GAGGATCTGGAGCAACTCGG |
| invF-RT-F | ACAACGCGAACATTCACACG |
| invF-RT-R | GCTGGGTGTTGGCAAATGAC |
| prgH-RT-F | TGGCCGACAATTACCCAGAC |
| prgH-RT-R | CGTTTGAGCTCCGTTTCTGC |
| prgI-RT-F | CCGGCGTTGAGGACTTACAA |
| prgI-RT-R | CGGAAGTTTTGTATGATGGCGG |
| sipA-RT-F | TTGACTCGACATTCTGCGCT |
| sipA-RT-R | CAAATCCGGCCACAAAGCTC |
| sipD-RT-F | GCCTGCCACGCTTGAAATTT |
| sipD-RT-R | TCAGTGATCGGAAGGTTGCC |
| sipC-RT-F | GCCTCAAGCGCAGACTTACT |
| sipC-RT-R | CTGGGGCAATTGATGGTCCT |
| sipB-RT-F | GCCTCAAGCGCAGACTTACT |
| sipB-RT-R | CTGGGGCAATTGATGGTCCT |
| spaT-RT-F | GCTTCCGCTTCATCGAGTCT |
| spaT-RT-R | GAAATGGTCTGGGAAGCGGT |
| spaP-RT-F | TGCTTTACCACCGGCATCAT |
| spaP-RT-R | GCGTCTGGGACCTGCTTTAT |
| invB-RT-F | CGAGCTCGCCGTCTTGAATA |
| invB-RT-R | AGCATTCACATCAGCCAGCA |
| invA-RT-F | AGTTAACCACGCGCACCATA |
| invA-RT-R | TCAAGGGCCACTGGAAAAGG |
| invE-RT-F | CCAACACCCGCTCAAAACTG |
| invE-RT-R | CAGCGTTTCGTTCAGTCTGC |
| invH-RT-F | ATGCAGCGAGGATGGAGTCA |
| invH-RT-R | ATGCCCGCTCAAATTCGCTA |
| hilA-RT-F | TACAAAATCGCATTGCCGCC |
| hilA-RT-R | AGCTGCAGTACAATGACGCT |
| iagB-RT-F | GCAACGTTATGGTTATAGCTGGG |
| iagB-RT-R | ACGTTCCCAAATTTTGTTAGCA |
| prgJ-RT-F | ATTACACGCCAAGACACCGAT |
| prgJ-RT-R | TCGAGGCGAGCTAAGATTGC |
| prgK-RT-F | AGTCGCCCAGATGTTTCCTG |
| prgK-RT-R | ACGCGGACAAGTGAATGGAT |
| orgA-RT-F | TCAACAACAAGCCGAGCAGA |
| orgA-RT-R | CATGGGCTAGCAACTGTCGA |
Underlined letters show Kpn1 、sacI、Xba1、BamhI or XhoI restriction site
F/R: The upstream and downstream primers
S/X-F/R: The upstream and downstream primers for the upstream and downstream gene fragments of rpoN or T2SS-2 in the O45 genome
U/D-F/R: Upstream and downstream sequencing primers of plasmid